Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system.

Charles Bruce Bagwell; Benjamin Hunsberger; Beth Hill; Donald Herbert; Christopher Bray; Thirumahal Selvanantham; Stephen Li; Jose C. Villasboas; Kevin Pavelko; Michael Strausbauch; Adeeb Rahman; Gregory Kelly; Shahab Asgharzadeh; Azucena Gomez‐Cabrero; Gregory Behbehani; Hsiaochi Chang; Justin Lyberger; Ruth Montgomery; Yujiao Zhao; Margaret Inokuma; Ofir Goldberger; Greg Stelzer

doi:10.1002/CYTO.B.21858

Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system.

Charles Bruce Bagwell
, Benjamin Hunsberger
, Beth Hill
, Donald Herbert
, Christopher Bray
, Thirumahal Selvanantham
, Stephen Li
, Jose C. Villasboas
, Kevin Pavelko
, Michael Strausbauch
, Adeeb Rahman
, Gregory Kelly
, Shahab Asgharzadeh
, Azucena Gomez‐Cabrero
, Gregory Behbehani
, Hsiaochi Chang
, Justin Lyberger
, Ruth Montgomery
, Yujiao Zhao
, Margaret Inokuma

Ofir Goldberger, Greg Stelzer

Verity Software House Topsham Maine
Fluidigm Canada Inc. Markham Ontario Canada
Mayo Clinic
Icahn School of Medicine at Mount Sinai
Children's Hospital Los Angeles
Ohio State University
Yale University
Fluidigm Corporation

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Cytometry Part B-clinical Cytometry
Volume	98
Issue number	2
DOIs	https://doi.org/10.1002/CYTO.B.21858
State	Published - Mar 1 2020
Externally published	Yes

Disciplines

Biology
Biological Engineering

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Bagwell, C. B., Hunsberger, B., Hill, B., Herbert, D., Bray, C., Selvanantham, T., Li, S., Villasboas, J. C., Pavelko, K., Strausbauch, M., Rahman, A., Kelly, G., Asgharzadeh, S., Gomez‐Cabrero, A., Behbehani, G., Chang, H., Lyberger, J., Montgomery, R., Zhao, Y., ... Stelzer, G. (2020). Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. Cytometry Part B-clinical Cytometry, 98(2). https://doi.org/10.1002/CYTO.B.21858

Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. / Bagwell, Charles Bruce; Hunsberger, Benjamin; Hill, Beth et al.
In: Cytometry Part B-clinical Cytometry, Vol. 98, No. 2, 01.03.2020.

Research output: Contribution to journal › Article › peer-review

Bagwell, CB, Hunsberger, B, Hill, B, Herbert, D, Bray, C, Selvanantham, T, Li, S, Villasboas, JC, Pavelko, K, Strausbauch, M, Rahman, A, Kelly, G, Asgharzadeh, S, Gomez‐Cabrero, A, Behbehani, G, Chang, H, Lyberger, J, Montgomery, R, Zhao, Y, Inokuma, M, Goldberger, O & Stelzer, G 2020, 'Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system.', Cytometry Part B-clinical Cytometry, vol. 98, no. 2. https://doi.org/10.1002/CYTO.B.21858

Bagwell CB, Hunsberger B, Hill B, Herbert D, Bray C, Selvanantham T et al. Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. Cytometry Part B-clinical Cytometry. 2020 Mar 1;98(2). doi: 10.1002/CYTO.B.21858

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author = "Bagwell, \{Charles Bruce\} and Benjamin Hunsberger and Beth Hill and Donald Herbert and Christopher Bray and Thirumahal Selvanantham and Stephen Li and Villasboas, \{Jose C.\} and Kevin Pavelko and Michael Strausbauch and Adeeb Rahman and Gregory Kelly and Shahab Asgharzadeh and Azucena Gomez‐Cabrero and Gregory Behbehani and Hsiaochi Chang and Justin Lyberger and Ruth Montgomery and Yujiao Zhao and Margaret Inokuma and Ofir Goldberger and Greg Stelzer",

note = "High-dimensional mass cytometry data potentially enable a comprehensive characterization of immune cells. In order to positively affect clinical trials and translational clinical research, this advanced technology needs to demonstrate a high reproducibility of results across multiple sites for both peripheral blood mononuclear cells (PBMC) and whole blood preparations.",

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AU - Herbert, Donald

AU - Bray, Christopher

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Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system.

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